The ddPCR method involves dividing nucleic acid samples into numerous microdroplets of known volume within a water-oil emulsion. Amplification of specific nucleic acid sequences occurs individually within each microdroplet using the same process and materials typically employed in a standard TaqMan® probe-based assay. Following amplification, fluorescence detection is used to categorize each droplet as either PCR-positive or PCR-negative. The concentration of target DNA/RNA templates (copies/µl) in the initial sample is then determined using a Poisson distribution based on the counted droplets.
